The selenium content of cell culture serum influences redox-regulated gene expression.
نویسندگان
چکیده
Cell Culture The MDA-MB-231 breast cancer cell line (see Reference 24 in the main text) was cultured in RPMI 1640 medium (Invitrogen, Melbourne, Australia), supplemented with either 10% FBS [either Invitrogen, Lonza (Melbourne, Australia), Quantum (Brisbane, Australia), or 10% serum supreme (Lonza) and 100 μg/mL penicillin (Invitrogen) and 100 μg/mL streptomycin (Invitrogen) in 5% CO2 at 37°C. Media used in transfections did not contain penicillin or streptomycin. All chemicals were purchased from SigmaAldrich (Castle Hill, Australia) unless otherwise specified.
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ورودعنوان ژورنال:
- BioTechniques
دوره 50 5 شماره
صفحات -
تاریخ انتشار 2011